• VPN|
  • 内网办公|
  • 财务报销|
  • 预约系统|
  • 科研信息管理系统(签章审批)|
  • 邮箱|
  • d88尊龙手机客户端|
  • 所长信箱|
  • English
MENU
d88尊龙手机客户端» 新闻动态» 最新论文

Development of a set of PCR markers specific to Aegilops longissima chromosome arms and application in breeding a translocation line

  作 者:Wang Kunyang, Lin Zhishan, Wang Long, Wang Ke, Shi Qinghua, Du Lipu, Ye Xingguo*

  影响因子:4.132

  刊物名称:Theoretic and Applied Genetics

  出版年份:2018

  卷:131  期:1   页码:13-25

  doi:10.1007/s00122-017-2982-5

  文章摘要 : 

  Aegilops longissimais an important wild species of common wheat that harbors many genes that can be used to improve various traits of common wheat (Triticum aestivum L.). To efficiently transfer the traits conferred by these Ae. longissima genes into wheat, we sequenced the whole expression transcript of Ae. longissima. Using the transcriptome data, we developed 134 specific polymerase chain reaction (PCR) markers located on the 14 chromosome arms of Ae. longissima. These novel molecular markers were assigned to specific chromosome locations based on a comparison with the homologous genes in the B genome of wheat. Annotation of these genes showed that most had functions related to metabolic processes, hydrolase activity, or catalytic activity. Additionally, we used these markers to identify three wheat-Ae. longissima 1BL?1SlS translocation lines in somatic variation populations resulting from a cross between wheat cultivar Westonia and a wheat-Ae. longissima substitution line 1Sl(1B). The translocation lines had several low molecular weight glutenin subunits (LMW-GSs) encoding genes beneficial to flour processing quality that came from Ae. longissima 1SlS. The three translocation lines were also confirmed by genomic in situ hybridization (GISH). These translocation lines will be further evaluated for potential quality improvement of bread-making properties wheat.

  下载链接:https://link.springer.com/content/pdf/10.1007/s00122-017-2982-5.pdf

  



TOP
XML 地图 | Sitemap 地图